Trigger Factor from the Psychrophilic Bacterium Psychrobacter frigidicola Is a Monomeric Chaperone
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چکیده
منابع مشابه
Trigger factor from the psychrophilic bacterium Psychrobacter frigidicola is a monomeric chaperone.
In eubacteria, trigger factor (TF) is the first chaperone to interact with newly synthesized polypeptides and assist their folding as they emerge from the ribosome. We report the first characterization of a TF from a psychrophilic organism. TF from Psychrobacter frigidicola (TF(Pf)) was cloned, produced in Escherichia coli, and purified. Strikingly, cross-linking and fluorescence anisotropy ana...
متن کاملTrigger Factor from the Psychrophilic Bacterium
16 17 In eubacteria, trigger factor (TF) is the first chaperone to interact with newly synthe-18 sised polypeptides and assist their folding as they emerge from the ribosome. We report the 19 first characterisation of a TF from a psychrophilic organism. TF from Psychrobacter frigidi-20 cola (TF PF) was cloned, produced in Escherichia coli and purified. Strikingly, cross-linking 21 and fluoresce...
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Reduced denatured lysozyme tends to aggregate at neutral pH and competition between productive folding and aggregation substantially reduces the efficiency of refolding. Trigger factor, a folding catalyst and chaperone can, depending on the concentration of trigger factor and the solution conditions, cause either a substantial increase (chaperone activity) or a substantial decrease (antichapero...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2009
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.01137-08